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MOLECULAR BIOLOGY LABORATORY PROFILE

DR TOPE SALAM

Welcome to the FIIRO Molecular Biology Laboratory, where innovation meets industrial advancement. Our laboratory is at the forefront of research, offering state-of-the-art molecular biology services designed to enhance the capabilities of the industrial sector. By integrating cutting-edge scientific techniques with a commitment to education and training, we are uniquely positioned to support both industry and academia in achieving their research and development goals.

Our Vision

We envision our lab as a hub of discovery and education, driving the integration of molecular biology into industrial processes and equipping the next generation of scientists with the tools to foster a more sustainable and prosperous future. Our goal is to set new standards in scientific innovation that empower the industrial sector to thrive globally.

Our Mission

Our mission is to leverage molecular biology to drive industrial innovation, research excellence, and capacity building across Nigeria. We aim to deliver world-class services that enhance the competitiveness and sustainability of Nigerian industries, while also providing top-tier training to empower life science researchers with the skills and knowledge necessary to excel in their fields.

Key Research Areas

Our laboratory focuses on several key areas of research that are critical to industrial development:

  1. Microbial Biotechnology for Industry: We explore and optimize microbial processes for industrial applications, including fermentation, bio-manufacturing, and environmental management.
  2. Genomic and Environmental Biotechnology: We apply genomic tools to tackle environmental challenges, promoting sustainable practices within the industrial sector.
  3. Quality Control: We develop molecular techniques for precise detection and identification of biological agents, ensuring high standards of product safety and quality in industrial production.
  4. Protein Engineering and Analysis: Our team enhances industrial enzymes and proteins, improving efficiency and performance in various manufacturing processes.

Description

The discovery of the DNA by Griffith et al in 1928 followed by the deciphering of its structure by Watson and Crick in 1953 revolutionized research in the life science. The understanding of the process of DNA replication also paved way for the development of the indispensable technique, polymerase chain reaction in molecular biology. The newly remodelled FIIRO Molecular Biology Laboratory is strategically positioned to offer world class molecular biology research and training services to the teeming Nigerian researchers and scientists. Our fully prepared and trained molecular biology team is ready to offer premium and top-notch molecular biology services to advance research in FIIRO as well as other internal and external clients. 

Services

Our services include but not limited to:

  1. Molecular Characterisation of Microorganisms.                   a. Polymerase Chain Reaction (PCR) Based Techniques.            b. Fluorescence Microscopy.
  2. DNA/RNA with cDNA Synthesis/Protein Extraction
  3. Quantification and Purity Assessment of Nucleic Acids
  4. Amplification of Target DNA Sequences.
  5. Mutation/Polymorphism Detection
  6. Protein Characterisation and Profiling.   a.Total Protein Profiling. b. Protein Characterisation. a. i. Molecular Weight Determination for Specific Proteins. b.ii. Identification of Specific Proteins.

Techniques

Some techniques to be used in service delivery include but not limited to:

  • PCR-RAPD
  • PCR-RFLP
  • PCR-ARDRA
  • PCR with Gene Specific Primers
  • 16SrRNA Sequencing
  • ITS Sequencing
  • Fluorescent in situ Hybridization (FISH)
  • Reverse Transcription-PCR (RT-PCR)
  • Semi Quantitative RT-PCR (SqRT-PCR)
  • Agarose Gel Electrophoresis
  • Temperature/Denaturing Gradient Gel Electrophoresis (DGGE/TGGE)
  • SDS-PAGE
  • Western Blotting

Acronyms

  • PCR: Polymerase Chain Reaction
  • RAPD: Randomly Amplified Polymorphic DNA
  • RFLP: Restriction Fragment Length Polymorphism
  • ARDRA: Amplified Ribosomal DNA Restriction Analysis
  • ITS: Internal Transcribed Spacer
  • SDS-PAGE: Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis 

Equipment

Some equipment are listed and shown below.

Autoclave          Bio-freezer   Dry-bath-1    Fluorescence-Microscope-1     Gel-Documentation-System-1    Gel-Dryer-1   Gradient-Thermal-Cycler-1        Horizontal-Electrophoresis-System    MicroDoc-with-Video-Processor       ThermoScientific-Nanodrop-2000c          UV-Transilluminator     Universal-Mutation-Detection-System      Vertical-Electrophoresis-with-Blotting-System       

 

Contact Person

For information on collaboration, bench space and sample analysis contact:

Dr Temitope O. Fadipe

Biotechnology Department

Molecular Biology Laboratory, Ground Floor, Koleosho Laboratory Complex, FIIRO, Lagos.

Email: This email address is being protected from spambots. You need JavaScript enabled to view it., This email address is being protected from spambots. You need JavaScript enabled to view it.

Mobile: +2348033183943

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