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Enzymes are biochemical catalysts produced by all living things. The most distinguishing property of an enzyme in its catalytic action is its specificity and selectivity, each enzyme catalyses specific substrate. Xylanases (EC 3.2.1) belong to the glucanase enzyme family and are characterized by their ability to breakdown various xylans to produce short-chain xylo-oligosaccharides. A total of 31 molds were isolated and thereafter screened for their ability to produce xylanase by using the plate assay technique on Potato Dextrose Agar fortified with 2% (w/v) of xylan. Promising mold isolates for xylanase production were identified as XA, XC₃, XD₂, XM₆ (Aspergillus niger), XC (Tricoderma harzianum), XC₁ (Fusarium oxysporum), XC₂, XM₂ (Aspergillus terreus), XD₃, XJ, XK (Aspergillus fumigatus), XM (Aspergillus tamarii). Xylanases were produced by Aspergillus niger and Tricoderma harzianum through solid state fermentation also, by Bacillus subtilis and Bacillus polymyxa through submerged fermentation process.Studies on the optimal conditions of performance of temperature and pH of the produced xylanases were in a range of 30⁰C - 80⁰C and 3.0 - 9.0 respectively. Comparative Studies of the enzyme for its activity as produced by isolate XM, XD, XC, A₂1 and A₂2 were 3000U/ml, 2800U/ml, 3400U/ml, 23241.70U/ml and 2940.00U/ml respectively. While the specific activity were 246.5U/mg/ml,97.15U/mg/ml,123.63U/mg/ml,2400.63U/mg/ml and 641.92U/mg/ml respectively.

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